557 Qualitative analysis of dermatophytosis in experimental in vivo and in vitro models relies on robust preparation of spores

Dermatophytoses are superficial infections of keratinized structures the host caused by filamentous fungi named dermatophytes. They most common mycosis worldwide, with an incidence estimated around 20% in humans and constant increase since last decade. Because this high growing emergence antifungal resistant strains, a better understanding mechanisms deployed dermatophytes to adhere on invade tissues is required for further identification therapeutic targets. Recently, several models have therefore emerged worldwide order study pathogenic steps involved during dermatophytosis. However, experimental procedures used grow condition as infectious spores were quite various, leading diverse composition inocula, thus rendering difficult overall interpretation data collected available models. By assessing various culture parameters, we identified growth potato dextrose agar at 30°C under 12% CO2 optimal improving sporulation viability dermatophyte species frequently isolated from human lesions, including Trichophyton rubrum benhamiae. The resulting suspensions characterized more homogenous composition, containing exclusively unicellular spores, sufficient amount use inoculum infection, like vivo mouse model, or vitro reconstructed epidermis (RHE). Furthermore, demonstrated infection RHE, these improves adhesion ability increases reproducibility keratinocyte responses. In conclusion, research paves way towards standardized procedure production inocula dermatophytosis, uniform results facilitating comparison observations between